Ⅰ. By binding ligand-gated ion channels, it opens cation channels.
Ⅱ. Defusing cation channels causes depolarization for generating action potential.
Ⅲ. A current of action potential is generated with the influx of Na+ and the efflux of K+.
Ⅳ. It directly affects the formation of synaptic vesicles for creating action potential.